Sometimes, yes. Some jurisdictions have transit (layover) rules that apply even if you are only connecting through an airport.
What PadsPass shows
When you plan a trip with connecting flights, PadsPass may show:
Origin requirements
Destination requirements
Transit (layover) country requirements (when they apply)
Example
If you fly from Mexico to Canada with a connection through the United States, you may see U.S. transit pet requirements in addition to Mexico and Canada requirements.
Important notes
Transit rules vary by country and can depend on your route, airline, whether you stay airside, and whether you need to clear immigration or re-check baggage.
Requirements can change, so always confirm current rules with your airline and the destination and transit authorities for your trip.
If you're unsure
If you're not sure whether your connection counts as “transit” for your situation, contact support and include:
Origin and destination
Layover airport(s) and country/countries
Travel dates
Pet type (dog, cat, service animal)